t cell activation/expansion kit, mouse (Miltenyi Biotec)

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Miltenyi Biotec t cell activation/expansion kit, mouse
T Cell Activation/Expansion Kit, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+cell+activation+expansion+kit/T+Cell+Activation%2FExpansion+Kit%2C+mouse/custom%40130-093-627%4042480527
Average 97 stars, based on 72 article reviews

t cell activation/expansion kit, mouse - by Bioz Stars, 2026-11

97/100 stars

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Isolation:

Article Title: Aiolos and Eos drive distinct human TH17 functional states.
Article Snippet: 489 Bulk TH17 cells were cultured in RPMI-1640 medium containing the following supplements: 490 1% (v/v) GlutaMAX-I, 1% (v/v) non-essential amino acids, 1 mM sodium pyruvate, 1% (v/v) L491 glutamine, 50 U/mL penicillin and streptomycin and 10% heat-inactivated FBS (all from Gibco, 492 life Technologies). .. Isolated TH17 cells (EasySep Hu TH17 Cell Enrichment kit II 493 STEMCELL#17862) were activated using the T Cell Activation/Expansion kit (Miltenyi #130- 494 091-441). .. To this end 1x106 cells/mL were activated at bead to cell ratio of 1:5 and 0,5 x105 ARTICLE IN PRESS AR TIC LE IN PR ES S ACCEPTED MANUSCRIPT 495 cells per well were seeded into round-bottom tissue culture treated 96-well plates for four days 496 when treated with Lenalidomide at a final concentration of 1uM (Sigma-Aldrich#SML2283) or 497 DMSO (SIGMA-Aldrich#D2438), otherwise specified.

Article Title: Metabolic characterization of the tumor microenvironment orchestrates therapeutic strategies and clinical outcomes in pancreatic cancer
Article Snippet: The cytotoxicity of effector cells against PDAC cells was assessed by measuring lactate dehydrogenase (LDH) release using a commercial kit (Cayman, 601170). .. Peripheral blood T cells were isolated from patients with PDAC using magnetic cell sorting (Miltenyi Biotec, 130-096-535) and pre-activated for 48 h with a T cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441). ..

Article Title: Aiolos and Eos drive distinct human TH17 functional states
Article Snippet: Bulk TH17 cells were cultured in RPMI-1640 medium containing the following supplements: 1% (v/v) GlutaMAX-I, 1% (v/v) non-essential amino acids, 1 mM sodium pyruvate, 1% (v/v) L-glutamine, 50 U/mL penicillin and streptomycin and 10% heat-inactivated FBS (all from Gibco, life Technologies). .. Isolated TH17 cells (EasySep Hu TH17 Cell Enrichment kit II STEMCELL#17862) were activated using the T Cell Activation/Expansion kit (Miltenyi #130–091–441). .. To this end 1 × 10 6 cells/mL were activated at bead to cell ratio of 1:5 and 0,5 × 10 5 cells per well were seeded into round-bottom tissue culture treated 96-well plates for four days when treated with Lenalidomide at a final concentration of 1uM (Sigma-Aldrich#SML2283) or DMSO (SIGMA-Aldrich#D2438), otherwise specified.

Activation Assay:

Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant
Article Snippet: .. T cells were then activated and expanded using the T Cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441, Germany) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (Umedium, 3022 A, China), IL-2 (Novoprotein, Shanghai, C013, China), IL-7 (Novoprotein, Shanghai, C013, China), and IL-15 (Novoprotein, Shanghai, CX47, China) for 7–10 days. .. The precipitated proteins were analyzed via SDS-PAGE and high-throughput mass spectrometry (Jingjie PTM BioLab Co., Ltd.) to identify interacting proteins.

Article Title: Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinoma
Article Snippet: .. To humanize the immune system, human pan T cells were expanded ex vivo using the T cell Activation/Expansion Kit (Miltenyi Biotech, Cat# 130–091-441). .. To humanize the immune system, human pan T cells were expanded ex vivo using the T cell Activation/Expansion Kit (Miltenyi Biotech, Cat# 130–091-441).

Article Title: Mapping cell type-resolved transcriptomic profiles to patient survival in pancreatic cancer.
Article Snippet: From the PBMCs, T cells were subsequently isolated by magnetic-activated cell sorting (Miltenyi Biotec, 130-096-535) in accordance with the manufacturer’s instructions. .. The purified T cells were then pre-activated for 48 h using a T cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441). .. Following QC, we prepared libraries using the 10× Genomics Chromium Next GEM Single Cell 3′ v3.1 protocol: nuclei were barcoded in GEMs via Chromium Controller, followed by cDNA recovery, PCR amplification, and purification with Qubit/Agilent QC.

Article Title: High-risk EBV promotes immune evasion in nasopharyngeal carcinoma by upregulating HLA-DP via the encoded BALF2-HR variant.
Article Snippet: Primary CD4+T cells (Miltenyi Biotec, 130-045-101, Germany) and CD8+T cells (Miltenyi Biotec, 130-045-201, Germany) were isolated from PBMCs using magnetic separation with CD4/CD8 microbeads. .. T cells were then activated and expanded using the T Cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441, Germany) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (Umedium, 3022A, China), IL-2 (Novoprotein, Shanghai, C013, China) IL-7 (Novoprotein, Shanghai, C013, China), and IL-15 (Novoprotein, Shanghai, CX47, China) for 7–10 days. .. T cells were then activated and expanded using the T Cell Activation/Expansion Kit (Miltenyi Biotec, 130-091-441, Germany) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (Umedium, 3022A, China), IL-2 (Novoprotein, Shanghai, C013, China) IL-7 (Novoprotein, Shanghai, C013, China), and IL-15 (Novoprotein, Shanghai, CX47, China) for 7–10 days.

Cell Culture:

Ex Vivo:

Purification:

FACS:

other:

Article Title: Overcoming claudin family homology: discovery of ARC101, a highly potent CLDN6-specific T-cell engager with a novel CD3 binder for ovarian adenocarcinoma.
Article Snippet: Female NSG (non-obese diabetic [NOD] severe immunodeficiency [SCID] gamma chain deficient (il2rg null)) mice (Jackson Laboratories, USA) aged 6–8 weeks and weighing 18–23 g were used.

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